anti cd81 primary antibody  (Proteintech)

Journal: Frontiers in Immunology

Article Title: Gemcitabine-cisplatin chemotherapy plus anti-PD-L1 therapy reinvigorates antitumor immune response by reprogramming the intrahepatic cholangiocarcinoma microenvironment

doi: 10.3389/fimmu.2025.1666393

Figure Lengend Snippet: Differential analysis of CD8 + T cells between untreated and GCP-treated ICC samples. (A) UMAP plots showing all T cells labeled in different colors according to cell type annotation. (B) Volcano plot showing the differentially expressed genes of intra-tumoral CD8 + T cells between pretreated and posttreated ICC samples. (C) Immunofluorescence images showing the infiltration of CD8 + CD81 + T cells in treatment-naïve ICC samples from the TMA cohort (n=89). Kaplan–Meier survival curves for OS (D) and RFS (E) of 89 ICC patients grouped by infiltration levels of CD8 + CD81 + T cells. P values were determined via log-rank test. (F) Boxplot showing infiltration levels of CD8 + CD81 + T cells in GCP-treated tumors revealed by multiplex immunofluorescence in GCP cohort. (G) Violin plots showing the expression scores of tissue resident, exhausted, and co-stimulatory gene signatures in tumor-infiltrating CD8 + T cells between pretreated and posttreated samples. The P values were calculated by Wilcox test. OS, overall survival; RFS, recurrence-free survival. ****p < 0.0001.

Article Snippet: After that, the slides were incubated with CD81 primary antibody (1:200 dilution, A01281-2, BOSTER) and Alexa Fluor 488 donkey anti-rabbit antibody as described above.

Techniques: Labeling, Immunofluorescence, Multiplex Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Enhance Chondrocyte Function by Reducing Oxidative Stress in Chondrocytes

doi: 10.3390/ijms26167683

Figure Lengend Snippet: Characterization of UCMSCs and UCMSC-EVs. ( A ) Phase-contrast image of UCMSCs cultured on the plastic culture plate. Scale bar: 100 μm. ( B ) Representative histograms showing the expression of CD105, CD90, CD73, CD45, CD34, and HLA-DR on the surface of UCMSCs. Unstained cells were included as negative controls to validate the specificity of antibody staining. ( C ) Flow cytometry analysis to quantify the percentage of UCMSC-EVs positive for CD9, CD63, and CD81. ( D ) Western blot analysis of the protein expressions of CD63, CD81, and α-tubulin in UCMSCs and UCMSC-EVs. ( E ) Morphology of UCMSC-EVs (arrowhead), as observed using TEM.

Article Snippet: Overnight incubation at 4 °C was conducted using primary antibodies against CD81 (Proteintech, Rosemont, IL, USA, 66866-1-lg; 1:1000), CD63, and α-tubulin.

Techniques: Cell Culture, Expressing, Staining, Flow Cytometry, Western Blot

Journal: Frontiers in Immunology

Article Title: Massively HIV-1-infected macrophages exhibit a severely hampered ability to differentiate into osteoclasts

doi: 10.3389/fimmu.2023.1206099

Figure Lengend Snippet: Expression of tetraspanins CD9 and CD81 during osteoclast differentiation and HIV infection. The expression level of CD9 and CD81 was measured using flow cytometry (mean fluorescence intensity per cell, or MFI) at 6 days post-infection (dpi) using both HIV inoculums. The results are representative of two independent experiments performed using cells from different donors (A) . Osteoclasts and their precursors at 6 dpi of HIV infection with the low-inoculum (upper panel) and control (non-infected) (lower panel) were stained with DAPI (for cell nuclei), PE (for HIV-p24 capsid antigen), FITC (for CD81), and merged using immunofluorescence staining and deconvolution microscopy to examine changes in the cellular localization of tetraspanins during maturation and HIV infection (x400) (B) . Osteoclasts and their precursors at 6 dpi of HIV infection with the low-inoculum showing CD81 expression (green) and HIV-infected giant multinuclear cells (red, with nuclei in blue). (C) Yellow arrows show perinuclear structures resembling viral-containing compartments (VCC). Scale bars: 20 μm. *<0.05 indicates a statistically significant difference.

Article Snippet: Cells detached by Accutase ® (StemCell Technologies, United States), were stained for surface antigens for 30 min at 4°C with the following antibodies: APC anti-human CD206 (cat550889), PE anti-human CD80 (cat566992), FITC anti-human HLA DR (cat555560), FITC anti-human CD9 and mouse anti-human CD81 primary antibody (cat555675) (from BD Biosciences, United States), Alexa Fluor 488 goat anti-mouse IgG H&L secondary antibody (ab150117) and APC Anti-CCR5 antibody (ab176536) (from Abcam, United States).

Techniques: Expressing, Infection, Flow Cytometry, Fluorescence, Staining, Immunofluorescence, Microscopy